Epidermal Mucus of Anabas testudineus as a Promising Source of Antibacterial and Anticancer Agents

Anabas testudineus is a sturdy freshwater fish that can live in polluted environment due to the epidermal mucus (EM) protects from pathogens or germs. This study explored functional properties of EM as potential antimicrobial and anticancer agent. Inactive Pseudomonas aeruginosa was introduced into tanks stimulate production EM. stimulus significantly increased by more than 100% after 10 days stimulation, indicating influenced environmental biotic stress. In vitro antibacterial activity tests showed has significant against Escherichia coli (12 ± 0.23 mm) P. (10 0.13 at tested concentration 1000 μg/mL. Further characterisation cells cytotoxic effect human breast cancer (MCF7) melanoma (A375.S2) producing an IC50 value 4.97 0.25 6.27 0.17 mg/mL, respectively. contrast, no cytotoxicity normal fibroblast skin (HS27) observed. addition, apoptosis analysis could cause DNA fragmentation cells, while on These findings indicated A. be further studied